02521nas a2200277 4500008004100000022001400041245012000055210006900175260001500244300001200259490000700271520171600278653001301994653001402007653002102021653000802042653001002050100001902060700001502079700001802094700001802112700001402130700001502144700001702159856006702176 2020 eng d a1323-893000aIsolation and characterization of the major centipede allergen Sco m 5 from Scolopendra subspinipes mutilans0 aIsolation and characterization of the major centipede allergen S c14/07/2020 a121-1280 v703 a

Background: Allergic reactions have been observed following both direct centipede bites and the clinical use of centipede-containing medicines, such as traditional Chinese medicines utilizing Scolopendra subspinipes mutilans; however, no natural centipede allergen has yet been characterized.

Methods: An allergen was purified from S. s. mutilans venom using Superdex 75 gel filtration and RESOURCE S ion chromatography, and its primary structure was determined via a combination of LC-MS-MS, MALDI-TOF/TOF and protein sequencing techniques. Its potential allergenicity was evaluated by immunoblotting, ELISAs, skin prick tests (SPTs) and mast cell activation assays.

Results: A novel allergen Sco m 5 (210 amino acids long) was successfully purified from crude S. s. mutilans venom. Sco m 5 could promote the degranulation of a human mast cell line, HMC-1. Among centipede-allergic patients, Sco m 5 showed an 83.3% IgE-binding frequency and a 66.7% positive reaction frequency, as detected by immunoblotting and SPTs, respectively. Sco m 5 IgE-binding frequencies of common Chinese population was found to be 9%–16%. Sera positive for Sco m 5 IgE-binding was cross-reactive against venom from the wasp Vespa mandarinia.

Conclusions: The present study isolated and characterized a novel allergen termed as Sco m 5 from the centipede S. s. mutilans. The use of Sco m 5 to identify centipede-allergic individuals could be important, given the high potential allergenicity of Sco m 5 among the general Chinese population, along with the likely possibility of cross-reactivity against wasp venom among centipede-allergic patients.

10aAllergen10aCentipede10aCross-reactivity10aIgE10aVenom1 aLan, Xin-Qiang1 aZhao, Feng1 aWang, Qi-Quan1 aLi, Jiang-Hua1 aZeng, Lin1 aZhang, Yun1 aLee, Wen-Hui uhttps://linkinghub.elsevier.com/retrieve/pii/S1323893020300927