02387nas a2200265 4500008004100000022002500041245015700066210006900223260001500292300001000307490000700317520151200324653002201836653001901858653002601877653002501903653002901928100002401957700001701981700002201998700001702020700001902037700001702056856004802073 2021 eng d a1314-2615, 1768-144800aDon’t forget the vertical dimension: assessment of distributional dynamics of cave-dwelling invertebrates in both ground and parietal microhabitats0 aDon t forget the vertical dimension assessment of distributional c14/10/2021 a43-630 v403 a

Biological studies on factors shaping underground communities are poor, especially those considering simultaneously organisms with different degrees of adaptation to cave life. In this study, we assessed the annual dynamics and use of both horizontal and vertical microhabitats of a whole community with the aim of understanding whether cave-dwelling organisms have a similar distribution among vertical and ground-level microhabitats and to find out which microhabitat features influence such distribution. We monthly assessed from 2017 to 2018, by direct observation combined with quadrat sampling method on the ground and transects on the walls, richness and abundance of 62 cave-dwelling species in a cave of Northern Italy. Environmental factors such as light intensity, temperature, relative humidity and mineralogical composition of the substrates were measured during each monitoring session, influencing the dynamics of the whole community and revealing significant differences between ground and wall microhabitats. A gradient of variation of the species assemblages occurred from the entrance toward inner areas, however, evidence that the dynamics of the walls are very different from those occurring at the ground independent from the distance from the surface are shown. Biodiversity indices highlighted sampling area diversity and a discrete total cave fauna biodiversity with the highest values found near the entrance and the lowest in the inner part of the cave.

10acave biodiversity10acave community10aenvironmental drivers10asubterranean biology10asubterranean environment1 aBalestra, Valentina1 aLana, Enrico1 aCarbone, Cristina1 aDe Waele, Jo1 aManenti, Raoul1 aGalli, Loris uhttps://subtbiol.pensoft.net/article/71805/02032nas a2200253 4500008004100000022001400041245012600055210006900181260001500250300001400265490000700279520124800286653001401534653002801548653001401576653001901590653001801609653001701627100001701644700002201661700002401683700002201707856004901729 2020 eng d a2037-463100aCentipede assemblages (Chilopoda) in high-altitude landforms of the Central-Eastern Italian Alps: diversity and abundance0 aCentipede assemblages Chilopoda in highaltitude landforms of the c31/08/2020 a1071-10870 v313 a

Centipedes (Chilopoda) are widespread and abundant predators in several kind of habitats, from forests to caves. Very few quantitative data are currently available for alpine habitats, specifically those located at high-altitudes. In this paper, we analysed data on centipedes collected by pitfall traps on different high-altitude landforms of the Central-Eastern Italian Alps, specifically on rock glaciers, debris-covered glaciers, glacier forelands, stable slopes, and scree slopes. We investigated the species richness and abundance (activity density) of individuals in each landform and the relationships between species occurrence and environmental variables in the investigated landforms. The obtained results highlighted non-significant differences in species richness between the landforms, but significant differences in individual abundance and species distribution. Soil organic matter, soil gravel percentage and altitude resulted the main variables affecting the species distribution; a clear preference for landforms located near the treeline was highlighted. Lithobius lucifugus resulted the dominant and most common species as well as the only species able to colonise debris-covered glaciers.

 

10aChilopods10aDebris-covered glaciers10amountains10aOrganic matter10aRock glaciers10aScree slopes1 aGobbi, Mauro1 aCaccianiga, Marco1 aCompostella, Chiara1 aZapparoli, Marzio uhttp://dx.doi.org/10.1007/s12210-020-00952-402624nas a2200229 4500008004100000022001400041245006100055210006100116260001500177300000900192490000800201520194600209653001902155653002702174653001802201653002302219653001902242100002002261700002402281700002202305856006702327 2020 eng d a0965-174800aDiversity and evolution of the P450 family in arthropods0 aDiversity and evolution of the P450 family in arthropods c22/10/2020 a1-220 v1273 a

The P450 family (CYP genes) of arthropods encodes diverse enzymes involved in the metabolism of foreign compounds and in essential endocrine or ecophysiological functions. The P450 sequences (CYPome) from 40 arthropod species were manually curated, including 31 complete CYPomes, and a maximum likelihood phylogeny of nearly 3000 sequences is presented. Arthropod CYPomes are assembled from members of six CYP clans of variable size, the CYP2, CYP3, CYP4 and mitochondrial clans, as well as the CYP20 and CYP16 clans that are not found in Neoptera. CYPome sizes vary from two dozen genes in some parasitic species to over 200 in species as diverse as collembolans or ticks. CYPomes are comprised of few CYP families with many genes and many CYP families with few genes, and this distribution is the result of dynamic birth and death processes. Lineage-specific expansions or blooms are found throughout the phylogeny and often result in genomic clusters that appear to form a reservoir of catalytic diversity maintained as heritable units. Among the many P450s with physiological functions, six CYP families are involved in ecdysteroid metabolism. However, five so-called Halloween genes are not universally represented and do not constitute the unique pathway of ecdysteroid biosynthesis. The diversity of arthropod CYPomes has only partially been uncovered to date and many P450s with physiological functions regulating the synthesis and degradation of endogenous signal molecules (including ecdysteroids) and semiochemicals (including pheromones and defense chemicals) remain to be discovered. Sequence diversity of arthropod P450s is extreme, and P450 sequences lacking the universally conserved Cys ligand to the heme have evolved several times. A better understanding of P450 evolution is needed to discern the relative contributions of stochastic processes and adaptive processes in shaping the size and diversity of CYPomes.

10aDetoxification10aEcdysteroid metabolism10aGene clusters10aOrthologous groups10aP450 Evolution1 aDermauw, Wannes1 aVan Leeuwen, Thomas1 aFeyereisen, René uhttps://linkinghub.elsevier.com/retrieve/pii/S096517482030179X02589nas a2200181 4500008004100000022001400041245013500055210006900190260001500259300000900274520195400283100002502237700001902262700002102281700002702302700002002329856005802349 2020 eng d a0748-300700aPhylogeny of Lithobiidae Newport, 1844, with emphasis on the megadiverse genus Lithobius Leach, 1814 (Myriapoda, Chilopoda)0 aPhylogeny of Lithobiidae Newport 1844 with emphasis on the megad c04/11/2020 a1-233 a

Phylogenetic analyses based on molecular and morphological data were conducted to shed light on relationships within the mostly Palaearctic/Oriental centipede family Lithobiidae, with a particular focus on the Palaearctic genus Lithobius Leach, 1814 (Lithobiidae, Lithobiomorpha), which contains >500 species and subspecies. Previous studies based on morphological data resolved Lithobius as nonmonophyletic, but molecular‐based phylogenetic analyses have until now sampled few species. To elucidate species inter‐relationships of the genus, test the validity of its classification into subgenera, and infer its relationships with other Lithobiidae, we obtained molecular data (nuclear markers: 18S rRNA, 28S rRNA; mitochondrial markers: 16S rRNA, COI) and 61 morphological characters for 44 species of Lithobius representing four of its eight subgenera and nine other representatives of Lithobiidae. The data were analyzed phylogenetically using maximum‐likelihood, parsimony and Bayesian inference. This study suggests that (i) a close relationship between L. giganteus and the pterygotergine Disphaerobius loricatus highlighted in recent morphological analyses is also strongly supported by molecular data, and Pterygoterginae is formally synonymized with Lithobiinae; (ii) the Oriental/Australian genus Australobius is consistently resolved as sister group to all other sampled Lithobiidae by the molecular and combined data; (iii) the subfamily Ethopolyinae may be paraphyletic; (iv) the genus Lithobius is nonmonophyletic; (v) the subgenera Lithobius, Sigibius and Monotarsobius are nonmonophyletic and should not be used in future taxonomic studies; and (vi) there are instances of cryptic species and cases in which subspecies should be elevated to full species status, as identified for some European taxa within Lithobius.

1 aGanske, Anne‐Sarah1 aVahtera, Varpu1 aDányi, László1 aEdgecombe, Gregory, D.1 aAkkari, Nesrine uhttps://onlinelibrary.wiley.com/doi/10.1111/cla.1243103914nas a2200505 4500008004100000022001400041245012800055210006900183260001500252300000900267490000700276520241600283653002402699653001302723653002302736653002102759653001802780653001802798653002102816653001202837653001902849100002702868700002002895700002202915700002102937700002202958700001802980700001702998700001703015700001803032700002303050700002003073700002303093700002403116700001803140700002403158700002803182700002703210700001903237700002003256700001403276700001903290700002103309856007803330 2020 eng d a1471-214800aFour myriapod relatives – but who are sisters? No end to debates on relationships among the four major myriapod subgroups0 aFour myriapod relatives but who are sisters No end to debates on c04/11/2020 a1-150 v203 a

Background: Phylogenetic relationships among the myriapod subgroups Chilopoda, Diplopoda, Symphyla and Pauropoda are still not robustly resolved. The first phylogenomic study covering all subgroups resolved phylogenetic relationships congruently to morphological evidence but is in conflict with most previously published phylogenetic trees based on diverse molecular data. Outgroup choice and long-branch attraction effects were stated as possible explanations for these incongruencies. In this study, we addressed these issues by extending the myriapod and outgroup taxon sampling using transcriptome data.

Results: We generated new transcriptome data of 42 panarthropod species, including all four myriapod subgroups and additional outgroup taxa. Our taxon sampling was complemented by published transcriptome and genome data resulting in a supermatrix covering 59 species. We compiled two data sets, the first with a full coverage of genes per species (292 single-copy protein-coding genes), the second with a less stringent coverage (988 genes). We inferred phylogenetic relationships among myriapods using different data types, tree inference, and quartet computation approaches. Our results unambiguously support monophyletic Mandibulata and Myriapoda. Our analyses clearly showed that there is strong signal for a single unrooted topology, but a sensitivity of the position of the internal root on the choice of outgroups. However, we observe strong evidence for a clade Pauropoda+Symphyla, as well as for a clade Chilopoda+Diplopoda.

Conclusions: Our best quartet topology is incongruent with current morphological phylogenies which were supported in another phylogenomic study. AU tests and quartet mapping reject the quartet topology congruent to trees inferred with morphological characters. Moreover, quartet mapping shows that confounding signal present in the data set is sufficient to explain the weak signal for the quartet topology derived from morphological characters. Although outgroup choice affects results, our study could narrow possible trees to derivatives of a single quartet topology. For highly disputed relationships, we propose to apply a series of tests (AU and quartet mapping), since results of such tests allow to narrow down possible relationships and to rule out confounding signal.

 

10aArthropod phylogeny10aconflict10aConfounding signal10aInternal rooting10aPhylogenetics10aphylogenomics10aQuartet topology10aRNA-Seq10aTranscriptomes1 aSzucsich, Nikolaus, U.1 aBartel, Daniela1 aBlanke, Alexander1 aBöhm, Alexander1 aDonath, Alexander1 aFukui, Makiko1 aGrove, Simon1 aLiu, Shanlin1 aMacek, Oliver1 aMachida, Ryuichiro1 aMisof, Bernhard1 aNakagaki, Yasutaka1 aPodsiadlowski, Lars1 aSekiya, Kaoru1 aTomizuka, Shigekazu1 aVon Reumont, Björn, M.1 aWaterhouse, Robert, M.1 aWalzl, Manfred1 aMeng, Guanliang1 aZhou, Xin1 aPass, Günther1 aMeusemann, Karen uhttps://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-020-01699-002214nas a2200265 4500008004100000022001400041245018400055210006900239260001500308300001000323490000800333520136200341653001401703653003201717653001501749653002501764100001501789700001901804700001901823700001601842700001201858700001601870700001501886856004701901 2020 eng d a1313-298900aThe complete mitochondrial genome sequence of Scolopendra mutilans L. Koch, 1878 (Scolopendromorpha, Scolopendridae), with a comparative analysis of other centipede genomes0 acomplete mitochondrial genome sequence of iScolopendra mutilansi c08/04/2020 a73-880 v9253 a

Scolopendra mutilans L. Koch, 1878 is an important Chinese animal with thousands of years of medicinal history. However, the genomic information of this species is limited, which hinders its further application. Here, the complete mitochondrial genome (mitogenome) of S. mutilans was sequenced and assembled by next-generation sequencing. The genome is 15,011 bp in length, consisting of 13 protein-coding genes (PCGs), 14 tRNA genes, and two rRNA genes. Most PCGs start with the ATN initiation codon, and all PCGs have the conventional stop codons TAA and TAG. The S. mutilans mitogenome revealed nine simple sequence repeats (SSRs), and an obviously lower GC content compared with other seven centipede mitogenomes previously sequenced. After analysis of homologous regions between the eight centipede mitogenomes, the S. mutilans mitogenome further showed clear genomic rearrangements. The phylogenetic analysis of eight centipedes using 13 conserved PCG genes was finally performed. The phylogenetic reconstructions showed Scutigeromorpha as a separate group, and Scolopendromorpha in a sister-group relationship with Lithobiomorpha and Geophilomorpha. Collectively, the S. mutilans mitogenome provided new genomic resources, which will improve its medicinal research and applications in the future.

10aChilopoda10aChinese medicinal materials10amitogenome10aScolopendra mutilans1 aHu, Chaoyi1 aWang, Shuaibin1 aHuang, Bisheng1 aLiu, Hegang1 aXu, Lei1 aHu, Zhigang1 aLiu, Yifei uhttps://zookeys.pensoft.net/article/47820/00339nas a2200109 4500008004100000245004300041210004300084300001200127490000700139100002900146856005400175 1878 eng d00aJapanesische Arachniden und Myriapoden0 aJapanesische Arachniden und Myriapoden a735-7980 v271 aKoch, Ludwig, Carl Chris uhttps://www.biodiversitylibrary.org/page/42882269