@article {1154, title = {Isolation and characterization of the major centipede allergen Sco m 5 from Scolopendra subspinipes mutilans}, journal = {Allergology International}, volume = {70}, year = {2020}, month = {14/07/2020}, pages = {121-128}, abstract = {

Background: Allergic reactions have been observed following both direct centipede bites and the clinical use of centipede-containing medicines, such as traditional Chinese medicines utilizing Scolopendra subspinipes mutilans; however, no natural centipede allergen has yet been characterized.

Methods: An allergen was purified from S. s. mutilans venom using Superdex 75 gel filtration and RESOURCE S ion chromatography, and its primary structure was determined via a combination of LC-MS-MS, MALDI-TOF/TOF and protein sequencing techniques. Its potential allergenicity was evaluated by immunoblotting, ELISAs, skin prick tests (SPTs) and mast cell activation assays.

Results: A novel allergen Sco m 5 (210 amino acids long) was successfully purified from crude S. s. mutilans venom. Sco m 5 could promote the degranulation of a human mast cell line, HMC-1. Among centipede-allergic patients, Sco m 5 showed an 83.3\% IgE-binding frequency and a 66.7\% positive reaction frequency, as detected by immunoblotting and SPTs, respectively. Sco m 5 IgE-binding frequencies of common Chinese population was found to be 9\%\–16\%. Sera positive for Sco m 5 IgE-binding was cross-reactive against venom from the wasp Vespa mandarinia.

Conclusions: The present study isolated and characterized a novel allergen termed as Sco m 5 from the centipede S. s. mutilans. The use of Sco m 5 to identify centipede-allergic individuals could be important, given the high potential allergenicity of Sco m 5 among the general Chinese population, along with the likely possibility of cross-reactivity against wasp venom among centipede-allergic patients.

}, keywords = {Allergen, Centipede, Cross-reactivity, IgE, Venom}, issn = {1323-8930}, doi = {10.1016/j.alit.2020.06.003}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1323893020300927}, author = {Lan, Xin-Qiang and Zhao, Feng and Wang, Qi-Quan and Li, Jiang-Hua and Zeng, Lin and Zhang, Yun and Lee, Wen-Hui} }