03109nas a2200313 4500008004100000022001400041245015900055210006900214260001500283300001200298490000700310520206800317653001402385653001202399653002102411653001402432653001902446100003502465700002102500700002002521700003202541700002202573700001902595700002302614700002502637700003302662700002902695856007102724 2020 eng d a2475-026300aInfluence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipede Lithobius forficatus (Myriapoda, Chilopoda)0 aInfluence of soil contaminated with cadmium on cell death in the c29/05/2020 a242-2620 v873 a

Cadmium is a heavy metal that is treated as an environmental pollutant (air, water, soil). In order to understand the potential effects of cadmium in soil and soil invertebrates, it is important to describe all alterations which appear at different levels in organisms. The main aim of this study was to investigate, analyze and describe the alterations caused by cadmium short- and long-term intoxication at different levels in the organisms: from tissues to cells and organelles. In addition, the activation of cell death mechanisms that take part in homeostasis maintenance according to cadmium has been studied. Therefore, as the species for this project, a terrestrial and well-known widespread European species – the centipede Lithobius forficatus (Myriapoda, Chilopoda, Lithobiomorpha) – was chosen. This omnivorous species lives under upper layers of soil, under stones, litter, rocks, and leaves, and it is also commonly found in human habitats. The animals were divided into three groups: C – the control group, animals cultured in a horticultural soil; Cd1 – animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 12 days – short-term exposure; Cd2 – animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, 45 days – long-term exposure. The midgut was isolated from each specimen and it was prepared for analysis using some histological, histochemical and immunohistochemical methods. Our studies showed that short-term intoxication causes intensification of autophagy and digestion of reserve material, while long-term exposure to this heavy metal causes activation of cell death processes together with inhibition of autophagy connected with the lack of reserve material. Additionally, we can infer that autophagy and cell death are nutrient deprivation-induced processes. Finally, we can conclude that short- and long-term exposure of soil centipede to cadmium affects different mechanisms and processes of cell death.

10aApoptosis10acadmium10aDigestive system10amyriapods10aUltrastructure1 aRost-Roszkowska, Magdalena, M.1 aPoprawa, Izabela1 aChajec, Łukasz1 aChachulska-Żymełka, Alina1 aWilczek, Grażyna1 aWilczek, Piotr1 aStudent, Sebastian1 aSkowronek, Magdalena1 aNadgórska-Socha, Aleksandra1 aLeśniewska, Małgorzata uhttps://www.tandfonline.com/doi/full/10.1080/24750263.2020.1757168