02468nas a2200289 4500008004100000022001400041245016100055210006900216260001500285300001400300490000700314520151200321653001301833653001401846653002301860653001901883653001401902653002501916100002601941700003101967700003001998700002202028700002502050700002002075700002702095856005602122 2019 eng d a0944-134400aHistopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides0 aHistopatology and HSP70 analysis of the midgut of iRhinocricus p c14/12/2019 a3023-30330 v273 a

Millipedes are organisms of the edaphic fauna and have been used as bioindicators for the evaluation of pollutants in the environment, as they are in constant contact with the soil. This study used the millipede Rhinocricus padbergi as surrogate to evaluate the toxicity of two metallic-insecticides that has been developed for leaf-cutting ants management. Millipedes were exposed in terrariums containing different concentrations of the metallic-insecticides and, after periods of 21 and 90 days, three individuals from each terrarium were dissected in order to remove the midgut, the organ where absorption of nutrients and, consequently, toxic substances occurs. The toxic action of the metallic-insecticides was analyzed through qualitative and semi-quantitative analysis of morphophysiological alterations and by quantitative analysis of the HSP70 stress protein. The results showed that the metallic-insecticides may increase HSP70 labeling, although not at all concentrations and periods of exposure. Histopathological alterations were not significant at any concentration, indicating that the cytoprotective action of HSP70 is able to prevent severe damage to the midgut. It is therefore suggested that the metallic-insecticides are not toxic to the species studied here as no toxicity was observed under the conditions tested. In addition, stress protein localization in midgut helps understand how morphophysiological processes can potentially be affected by pesticide exposure.

10adiplopod10aFormicide10aHeat shock protein10aHistochemistry10aHistology10aImmunohistochemistry1 ade Souza, Raphael, B.1 aMoreira-de-Sousa, Cristina1 aAnsoar-Rodríguez, Yadira1 aCoelho, Maria, PM1 ade Souza, Cleiton, P1 aBueno, Odair, C1 aFontanetti, Carmem, S. uhttp://link.springer.com/10.1007/s11356-019-07203-6